Evaluation the safety and synergistic effect of NiFe2O4 nanoparticles with antibiotic against Pseudomonas aeruginosa

The antimicrobial resistance currently impedes and threatens the future of effective prevention treatment continually expanding range infections caused by bacteria. This study aimed to identify bacterial causes wound infection among animals using antibiotic/nanoparticles mixture as a new attempt for induced in rats. For this purpose, 112 swabs cases different animal types (36 sheep, 21 goats, 12 cows, 4 horses, 8 dogs, 9 rabbits, 7 genies pigs 15 rats) were studied isolation. Pseudomonas aeruginosa was tested its sensitivity antibiotics nanoparticles (CoFe2O4 NiFe2O4) vitro MIC method. Also rats effect nanoparticles/antibiotics vivo. results showed that P. predominant type infection. minimum inhibitor concentration NiFe2O4 CoFe2O4 32 µg /ml 16 respectively. A clear synergistic antibiotic/ antibacterial noticed which appear decrease increase inhibitory diameter zone. According result Random Amplification Polymorphic DNA test, effects on genetic material observed an appearance/disappearance bands, thickness clarity bands.